Live imaging looks deeper

Live imaging looks deeper

Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.

Digging deeper with CT imaging: slice-by-micro slice...

Second-order optical coherence tomography: deeper and turbulence-free imaging

The superior resolution of optical coherence tomography (OCT) with respect to alternative imaging modalities makes it highly attractive, and some of its applications are already in extensive clinical use. However, one of the major limitations of OCT is that the tomographic picture it generates is depth-limited to approximately 1 mm in most biological tissues. This is mainly due to the spatially...

Live cell imaging of plants.

Live cell imaging is an essential approach for studying the structure, dynamics, and functions of cells in a living plant under normal or stressed growth conditions. The tiny flowering plant, Arabidopsis thaliana, provides an ideal system to apply various live microscopy techniques. Its small size allows fluorescent light to penetrate the tissues, and its plantlets contain different cell types ...

Live imaging of chromosome dynamics.

Progression of meiosis has been traditionally reconstructed from microscopic images collected from fixed cells. However, studies conducted in a number of species, including plants, indicate that this approach has clear shortcomings in accurately portraying the dynamic nature of meiotic processes. Here, we describe two methods to study chromosome dynamics in live meiocytes in maize, a protocol t...